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Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells. Methods used for the identification of spliced peptides on tumors are also described.

Original publication





Publication Date





159 - 186


Ludwig Institute for Cancer Research, Brussels, Belgium.


T-Lymphocytes, Cytotoxic, COS Cells, Cell Line, Tumor, Animals, Humans, Neoplasms, Peptides, Tumor Necrosis Factor-alpha, DNA, Complementary, Antigens, Neoplasm, Chromatography, High Pressure Liquid, Lymphocyte Activation, Antigen Presentation, Alternative Splicing, Amino Acid Sequence, Phosphorylation, Gene Library, Chromatography, Reverse-Phase, HEK293 Cells, Chlorocebus aethiops