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Whether cell types exposed to a high level of environmental insults possess cell type-specific prosurvival mechanisms or enhanced DNA damage repair capacity is not well understood. BRN2 is a tissue-restricted POU domain transcription factor implicated in neural development and several cancers. In melanoma, BRN2 plays a key role in promoting invasion and regulating proliferation. Here we found, surprisingly, that rather than interacting with transcription cofactors, BRN2 is instead associated with DNA damage response proteins and directly binds PARP1 and Ku70/Ku80. Rapid PARP1-dependent BRN2 association with sites of DNA damage facilitates recruitment of Ku80 and reprograms DNA damage repair by promoting Ku-dependent nonhomologous end-joining (NHEJ) at the expense of homologous recombination. BRN2 also suppresses an apoptosis-associated gene expression program to protect against UVB-, chemotherapy- and vemurafenib-induced apoptosis. Remarkably, BRN2 expression also correlates with a high single-nucleotide variation prevalence in human melanomas. By promoting error-prone DNA damage repair via NHEJ and suppressing apoptosis of damaged cells, our results suggest that BRN2 contributes to the generation of melanomas with a high mutation burden. Our findings highlight a novel role for a key transcription factor in reprogramming DNA damage repair and suggest that BRN2 may impact the response to DNA-damaging agents in BRN2-expressing cancers.

Original publication

DOI

10.1101/gad.314633.118

Type

Journal article

Journal

Genes Dev

Publication Date

01/03/2019

Volume

33

Pages

310 - 332

Keywords

BCL2, BRN2, Ku80, POU3F2, apoptosis, melanoma, nonhomologous end-joining, somatic mutation burden, Apoptosis, Cell Line, Tumor, DNA End-Joining Repair, Gene Expression Regulation, Neoplastic, Homeodomain Proteins, Humans, Ku Autoantigen, Melanoma, Mutation, POU Domain Factors, Poly (ADP-Ribose) Polymerase-1, Protein Binding, Protein Domains, Protein Transport