Genetic screens in human cells using RNA-sequencing
5 August 2016
Reverse genetics - the approach of understanding gene function by analysing the effects of altered DNA sequences - has been used on a large scale to ‘label’ genes with particular functions in model organisms, but it is technically difficult in human cells. Sebastian Nijman and colleagues now present a scalable approach to link genotype to phenotype in human cells, which could be used to study poorly characterised human genes.