Multiparameter persistent homology landscapes identify immune cell spatial patterns in tumors
Vipond O., Bull JA., Macklin PS., Tillmann U., Pugh CW., Byrne HM., Harrington HA.
Significance Quantifying and comparing complex spatial biological datasets is crucial for medical applications and remains an active area of research. As datasets become more heterogeneous and complicated, so must the methods that are used to understand them. Multiparameter topology is built upon the assumption that the shape of data depends on multiple parameters, such as scale, outliers, or other parameters (e.g., cell density and oxygen levels in the case of tumors). A key difficulty encountered in multiparameter persistent homology (MPH) is interpreting and comparing data. The present work uses statistical MPH landscapes to overcome this difficulty and quantifies differences in synthetic data of immune cell infiltration as well as clinical tumor histology data of T cells, macrophages, and hypoxia.