Figure 2 from Oncogenic Cell Tagging and Single-Cell Transcriptomics Reveal Cell Type–Specific and Time-Resolved Responses to <i>Vhl</i> Inactivation in the Kidney

<p>scRNA-seq on flow-sorted renal cells. <b>A,</b> UMAP plot of tdTomato-negative (left) or tdTomato-positive cells (right) from kidneys of Control mice harvested at the early time point. Cells are colored by inferred cell type. LoH, loop of Henle; DCT, distal convoluted tubule; CD, collecting duct; PC, principal cell; IC, intercalated; PEC, parietal epithelial cell; VSMC, vascular smooth muscle cell; NK, natural killer cell. <b>B,</b> Proportion of sequenced cells inferred to be of each cell type in tdTomato-positive or tdTomato-negative populations from kidneys of Control mice harvested at the early time point. Median and interquartile range plotted. <b>C,</b> UMAP plot depicting expression of PT Module A (left) and PT Module B (right) genes in PT cells from Control mice. <b>D,</b> Representative <i>in situ</i> RNA hybridization exhibiting spatially distinct expression of <i>Neat1</i> (blue) and <i>Fxyd2</i> (red) mRNA in FFPE kidney cortex from Control mice harvested at the early time point. Scale bar, 10 μm. Magnification, ×40. <b>E,</b> UMAP plot depicting cells from Control mice at the early time point. Cells are colored by assigned PT Class. <b>A–E,</b> scRNA-seq data shown for <i>n</i> = 3 female (3F) and <i>n</i> = 1 male (1M) Control mice.</p>