Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate
Yuan F., Bi Y., Siejka-Zielinska P., Zhou Y-L., Zhang X-X., Song C-X.
WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm5C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m5C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).