Joana Carrelha
Flow Cytometry Lead
research interests
Flow cytometry is a laboratory technology that allows the rapid profiling of large numbers of single cells and other particles. It is used both in basic research and as a clinical diagnostic tool.
My role is to manage and maintain the Ludwig flow cytometry instruments (cell analysers and cell sorters), to train researchers in instrument operation, and to provide technical and scientific support to all flow cytometry experiments in the Ludwig.
As a member of the Lu lab, I will lend my flow cytometry and cell biology expertise to develop and support the projects in the Cancer Grand Challenges award.
background
I have a MSc in Molecular Biology and Genetics from the University of Lisbon and a DPhil in Clinical Laboratory Sciences from the University of Oxford. I was a member of the Sten Eirik Jacobsen lab at the Weatherall Institute of Molecular Medicine (WIMM) for 12 years, first as a DPhil student and Marie Curie Early Stage Researcher in the EuroCancer Stem Cell Training Network, and then as a Postdoctoral Scientist. My research focused on the molecular and functional heterogeneity of haematopoietic stem and progenitor cells (HSPCs) in the bone marrow, and I specialised in high parameter flow cytometry and fluorescence-activated cell sorting. Afterwards, I was part of the Department of Immunology and Inflammation at Imperial College London for 2.5 years, first as Flow Cytometrist in a joint platform with the MRC Laboratory of Medical Sciences (LMS) and then as Flow Cytometry Facility Manager of a newly established facility.
Recent publications
Autophagy preserves hematopoietic stem cells by restraining MTORC1-mediated cellular anabolism.
Journal article
Borsa M. et al, (2024), Autophagy, 20, 45 - 57
Epigenetic programming defines haematopoietic stem cell fate restriction
Journal article
Meng Y. et al, (2023), Nature Cell Biology, 25, 812 - 822
Cross-Talk between Hematopoietic Cells and Fibroblast Subsets Drives Inflammation and Remodelling of the Bone Marrow Microenvironment in Myeloproliferative Neoplasms
Conference paper
Li R. et al, (2022), Blood, 140, 1091 - 1092
Ezh2 and Runx1 Mutations Collaborate to Initiate Lympho-Myeloid Leukemia in Early Thymic Progenitors
Journal article
Booth CAG. et al, (2018), Cancer Cell, 33, 274 - 291.e8
Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells
Journal article
Carrelha J. et al, (2018), Nature, 554, 106 - 111
