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Lysates from E. coli expressing HIV-1 reverse transcriptase (RT) as a TrpE fusion protein were used for immunization of BALB/c mice. Twenty hybridomas producing monoclonal antibodies (MAbs) recognizing the RT part of the TrpE-RT fusion protein by Western blot analysis were isolated. Of these, 18 were reactive in immunofluorescence assays when tested on HIV-infected cells. Twelve MAbs were reactive with both the p66 and p51 fragments of RT, while 6 of the MAbs were reactive only with the p66 band, indicating specificity for the C-terminal (RNase H) region of RT. Mapping of the monoclonal antibody binding sites was performed using deletion and insertion mutants of recombinant RT. The antibodies bound to five distinct regions within amino acid sequences 190-560 of RT. In order to map functionally important regions of the RT molecule, the MAbs were tested for their ability to interfere with the polymerase and RNase H activities of the polypeptide. MAbs binding to two different epitopes in the polymerase domain were found to inhibit the polymerase activity. Of these, three MAbs also inhibited the RNase H activity. Two MAbs binding to the same epitope in the RNase H region inhibited RNase H activity and further mediated an effect on the polymerase activity.

Type

Journal article

Journal

J Acquir Immune Defic Syndr

Publication Date

1992

Volume

5

Pages

647 - 657

Keywords

Amino Acid Sequence, Animals, Antibodies, Monoclonal, Binding Sites, Antibody, Binding, Competitive, Epitopes, HIV Reverse Transcriptase, HIV-1, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptide Mapping, RNA-Directed DNA Polymerase, Reverse Transcriptase Inhibitors, Ribonuclease H